Fig. 4

Antibody permeability and accumulation dynamics within an in vitro metastatic blood-tumor barrier model. a Representative images of antibody permeability with and without cancer cells. Images are at 30 min after perfusion with non-specific or anti-HER2 IgG in BBB and BTB microvessels. iBMECs (red), non-specific IgG (blue), anti-HER2 IgG (magenta), JIMT-1-BR (green). b Quantification of focal leaks between BBB and BTB microvessels over time (n = 4–5 independent microvessels per condition). c Representative images of antibody accumulation within the BBB and BTB at day 4. Images are normalized to day 2 fluorescence. d Quantification of antibody accumulation across BBB endothelium, BTB endothelium, and cancer spheroids (n = 4 independent microvessels per condition). Data are presented as mean ± SD. *p < 0.05. See also Additional file 1: Fig. S2