Fig. 9
From: Neurovascular unit disruption and blood–brain barrier leakage in MCT8 deficiency
Angiogenesis-related genes expression analysis by qRT-PCR in the cerebral cortex of WT and Mct8/Dio2KO mice. A Expression of the main BBB-associated proangiogenic genes at E15.5 and E18.5: Vegfa, Fgf2, Eng and Wnt7a. B Expression of the main BBB-associated antiangiogenic genes at E15.5 and E18.5: Angpt2, Serpinf, Fbln7, and Col18a1. n = 6 for each experimental group at E15.5, n = 5 for E18.5 WT embryos and n = 6 for E18.5 Mct8/Dio2KO embryos. Vegfa (VEGFA, vascular endothelial growth factor type A), Fgf2 (bFGF, fibroblast growth factor 2), Eng (endoglin), Wnt7a (WNT7A, wingless-type MMTV integration site family, member 7A), Angpt2 (ANG-2, angiopoietin 2), Serpinf (PEDF, pigment epithelium-derived factor), Fbln7 (fibulin-7), Col18a1 (endostatin, proteolytic fragment of the collagen type XVIII protein), FC fold changes. Data are expressed as bar plots with individual values, mean ± SD. P-values *p < 0.05, **p < 0.01, and ***p < 0.001 were determined by unpaired Student’s t-test or Mann–Whitney’s test