Fig. 5
From: Neurovascular unit disruption and blood–brain barrier leakage in MCT8 deficiency
Analysis of acute and sub-acute brain microhemorrhages in WT and Mct8/Dio2KO mice brain. A Representative images of the H&E-stained brain sections from WT and Mct8/Dio2KO mice at P90 and P180 showing an acute microhemorrhage. Black arrows point at red blood cells leaking from a blood vessel in the Mct8/Dio2KO mice at both ages. The graph to the right displays a quantification of the number (#) of acute microhemorrhages stained by H&E in the whole brain parenchyma of WT and Mct8/Dio2KO mice at P90 and P180. B Representative images of the Prussian Blue-stained brain sections from WT and Mct8/Dio2KO mice at P90 and P180 showing a sub-acute microhemorrhage. The graph to the right displays a quantification of the number (#) of sub-acute microhemorrhages stained by Prussian Blue in the whole brain parenchyma of WT and Mct8/Dio2KO mice at P90 and P180. Scale bar: 50 µm for panel A and 12.5 µm for panel B. n = 4 for each experimental group. Data are expressed as bar plots with individual values, mean ± SD. P-values *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 were determined by unpaired Student’s t-test