Fig. 4

Evaluation of the infiltration of non-permeable dyes in the brain of WT and Mct8/Dio2KO mice. A Schematic representation of the protocol for Evans Blue (EB) and sodium fluorescein (SF) infiltration analysis. B Colorimetric quantification of the EB extracted from brains of WT and Mct8/Dio2KO mice at P90 and P180. For EB procedures, WT animals: n = 8 at P90 and n = 5 at P180; Mct8/Dio2KO animals: n = 5 at P90 and n = 8 at P180. C Fluorimetric quantification of the SF extracted from brains of WT and Mct8/Dio2KO mice at P90 and P180. For SF procedures, WT animals: n = 9 at P90 and n = 7 at P180; Mct8/Dio2KO animals: n = 7 at P90 and n = 6 at P180. D Relative brain water content of the brains of WT and Mct8/Dio2KO mice at P90 and P180. For brain water content calculations, WT animals: n = 14 at P90 and n = 8 at P180; Mct8/Dio2KO animals: n = 12 at P90 and n = 6 at P180. EB  Evans Blue, SF  sodium fluorescein, AU  arbitrary units. Data are expressed as bar plots with individual values, mean ± SD. P-values *p < 0.05, and **p < 0.01 were determined by unpaired Student’s t-test or Mann–Whitney’s test