Fig. 3

Immunodetection of IgG infiltration in the cerebral cortex of P90 and P180 WT and Mct8/Dio2KO mice. A, B Representative microphotographs showing IgG immunodetection in the motor cortex of WT and Mct8/Dio2KO mice at P90 (A) and P180 (B). C Quantification of IgG staining intensity in the motor cortex of WT and Mct8/Dio2KO mice at P90 and P180. D, E Representative microphotographs showing IgG immunodetection in the somatosensory cortex of WT and Mct8/Dio2KO mice at P90 (D) and P180 (E). F Quantification of IgG staining intensity in the somatosensory cortex of WT and Mct8/Dio2KO mice at P90 and P180. For all panels, left images show low magnification photographs of general parenchyma staining and right images show high magnification images of blood vessels. Black arrows point at IgG-leaking blood vessels. Scale bar: 50 µm and 20 µm for low and high magnification images, respectively. n = 4 for each experimental group of animals. FC  fold change. Data are expressed as bar plots with individual values, mean ± SD. P-values *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 were determined by unpaired Student’s t-test