Table 1 List of study antibodies with functionality and origin
Primary antibody | Usage of antibody | Dilution | Species | Source |
|---|---|---|---|---|
Anti-Sortilin (cargo receptor antibody) | Transport experiment Immunocytochemistry Immunohistochemistry Western blotting | 10 µg/ml 10 µg/ml 8 µg/ml 1 µg/ml | Mouse | In-house developed |
Anti-CD133 (cargo receptor antibody) | Transport experiment Immunocytochemistry Immunohistochemistry Western blotting | 10 µg/ml 10 µg/ml 25 µg/ml 1 µg/ml | Mouse | DSHB clone HB#7 |
Anti-Podocalyxin (cargo receptor antibody) | Transport experiment Immunocytochemistry Immunohistochemistry Western blotting | 10 µg/ml 10 µg/ml 1.25 µg/ml 1 µg/ml | Mouse | R&D Systems MAB1658 |
Anti-B12/TfR (cargo receptor antibody) | Transport experiment Immunocytochemistry Western blotting | 10 µg/ml 10 µg/ml 1 µg/ml | Humanized mouse antibody | In-house developed from Genentech [45] |
Anti-B12/Sortilin (cargo receptor antibody) | Transport experiment Immunocytochemistry Western blotting | 10 µg/ml 10 µg/ml 0.8 µg/ml | Humanized mouse antibody | In-house developed from Genentech [45] |
Anti-B12/TfR-647 | Immunohistochemistry | 27 µg/ml | Humanized mouse antibody | In-house developed from Genentech [45] |
Anti-TNP (Trinitrophenol) Control antibody | Transport experiment Immunocytochemistry | 10 µg/ml 10 µg/ml | Humanized mouse antibody | In-house developed |
Anti-EEA1 | Immunocytochemistry | 13 µg/ml | Rabbit | Abcam ab109110 |
Anti-Rab7 | Immunocytochemistry | 5 µg/ml | Rabbit | Abcam ab137029 |
Anti-TGN46 | Immunocytochemistry | 2.5 µg/ml | Sheep | AbD-Serotec AHP500GT |
Anti-Rab8 | Immunocytochemistry | 4.4 µg/ml | Rabbit | Cell signaling CST-6975 T |
Anti-ZO1 | Immunocytochemistry | 6.5 µg/ml | Rabbit | Lundbeck, Denmark |
Anti-Human Fc | ELISA | 500 ng/ml | Goat | Sigma I2136 |
Anti-Mouse Fc | ELISA | 500 ng/ml | Goat | Sigma M2650 |
Anti-HRP-Human Fc | ELISA | 1 µg/ml | Goat | Sigma A0170 |
Anti-HRP-Mouse Fc | ELISA | 1 µg/ml | Goat | Sigma A0168 |
Anti-Mouse Alexa488 | Immunocytochemistry | 10 µg/ml | Donkey | Invitrogen A21202 |
Anti-Human Alexa488 | Immunocytochemistry | 10 µg/ml | Donkey | Biotium 13C0308 |
Anti-Sheep Alexa568 | Immunocytochemistry | 10 µg/ml | Donkey | Molecular probes A21099 |
Anti-Rabbit StarRed | Immunocytochemistry | 5 µg/ml | Goat | Abberior STRED-1002 |