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Fig. 5 | Fluids and Barriers of the CNS

Fig. 5

From: Subcellular trafficking and transcytosis efficacy of different receptor types for therapeutic antibody delivery at the blood‒brain barrier

Fig. 5

Bispecific cargo antibody hitchhiking on sortilin shows intracellular accumulation. Bar plots show colocalization scores with sums (A) and transcytosis (B) analysis using 10 µg/ml bispecific human anti-B12/sortilin construct on hiPSC-BECs. The bar plot in (C, D) shows the equivalent analysis for Caco-2 cells. A humanized control antibody targeting trinitrophenol (TNP, not expressed by mammals) was used as a parallel control (B, E). TNP was also tested by immunofluorescence staining, which showed no binding or internalization into the examined cell layers. Statistical significance was determined using ordinary two-way ANOVA test based on three independent experiments

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Fluids and Barriers of the CNS

ISSN: 2045-8118