Fig. 4

Transcytosis of cargo antibodies through cell barriers. A ELISA measurement of cargo antibody in the Transwell bottom acceptor chamber in relation to the added antibody in the top donor chamber given as transcytosed IgG percentage through the hiPSC-BEC layer. The red stippled line indicates the lower level of detection (approx. 50 pM) for the ELISA setup. B ELISA quantification of cargo antibody transcytosis through the Caco-2 cell layer. Statistical significance was determined using two-way ANOVA with Tukey´s multiple comparisons test based on four independent experiments. See also Additional file 2: Fig S5