Fig. 3From: In vitro investigation of the effect of proinflammatory cytokines on mouse choroid plexus membrane transporters Ncbe and NKCC1Effect of the proinflammatory cytokine TNFα on the CP expression of NKCC1, pNKCC1 and pSPAK. Primary cultures were treated with either 1 pg/mL, 1 ng/mL, or 100 ng/mL TNFα for 24 (A) or 48 (B) hours. Cells were lysed and subjected to immunoblotting for NKCC1, pNKCC1, pSPAK and proteasome 20 S on the same membrane. Molecular masses (shown in kDa) are indicated on the left. The NKCC1, pNKCC1, pSPAK and proteasome 20 S specific bands were quantified densiometrically. Bargraphs in (C) and (D) show the ratio of NKCC1/proteasome 20 S (left), pNKCC1/proteasome 20 S (middle), and pSPAK/proteasome 20 S (right) as mean ± SEM. *indicates p < 0.05 and **p < 0.01Back to article page