Fig. 6

Immuno-SEM of meninges. Sections of normal mouse spinal meninges adhered to adhesive tape, and processed for immuno-SEM, or immunofluorescence/confocal microscopy as described in Methods. (a) Immuno-SEM of collagen I staining (backscatter mode), showing 1 nm gold labeling (white dots) localized to the bony vertebrae. (b) Immunofluorescence of collagen I exclusively staining the bony vertebrae overlying the meninges; the dura and arachnoid membranes show intense pancytokeratin staining. (c) Immuno-SEM of pancytokeratin staining (backscatter mode), showing 1 nm gold staining (white dots) of what appear to be trabecular sheets (arrows); gold labeling can be seen extending toward the arachnoid membrane. (d) Immunofluorescence of pancytokeratin staining, highlighting similar sheets of trabeculae as seen in SEM, as well as arachnoid and pial membranes. (e) Low magnification immuno-SEM (secondary electron mode) of collagen II staining, highlighting sheet-like forms of trabeculae. (f) High magnification immuno-SEM of collagen II staining (backscatter mode), showing enlarged region (red box) in (e) containing 1 nm gold labeling (white dots)