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Fig. 4 | Fluids and Barriers of the CNS

Fig. 4

From: Proteomic interrogation of the meninges reveals the molecular identities of structural components and regional distinctions along the CNS axis

Fig. 4

Immunofluorescence detection of trabeculae. (a) SEM of rat meninges, performed as described (Saboori, [111]), highlighting reticular network of diverse trabeculae. (b)Immunofluorescence of mouse spinal meninges, carried out on a tissue section cut from intact mouse spinal column and transferred to adhesive tape as described in Methods. Structural features of spinal meninges align with near superimposition to those in the SEM, highlighting staining of trabeculae by anti-collagen II and pan-cytokeratin antibodies. (c) Collagen II staining of mouse spinal meninges highlights plate-like (✻), tree-like ( ←), veil-like (✭), and rod-like (◀) trabeculae seen in SEM (Saboori, [111]), as well as the dura. (d) Mouse optic nerve meninges indicating non-overlapping collagen I and collagen II staining

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Fluids and Barriers of the CNS

ISSN: 2045-8118