Fig. 3

Transcriptional changes of LOAD BCECs co-cultured with isogenic cell types. Comparison of control (CON) and late-onset Alzheimer disease (LOAD) neurovascular unit models by high-throughput multiplex qRT-PCR analysis of blood-brain barrier markers expressed in BCECs (n = 35 independent differentiations). (A) The value of the logarithm of fold change (Log₂FC) for each transcript is plotted on a heat map indicating up- (red) and down-regulated (blue) transcripts (Log₂FC > 0 and < 0, respectively). (B) Volcano plot displaying Log₂FC and -Log₁₀ (p-values) for each transcript. The Log₂FC = -1/1 and the p = 0.05 are indicated (grey lines). (C) List of significantly regulated blood-brain barrier markers (mean + SEM; Student’s t-test, p ≤ 0.05). (D) Venn diagram illustrating the number significantly regulated transcripts shared between different brain cell types. BCECs, brain capillary endothelial-like cells; ACs, astrocytes; MGCs, microglia-like cells; NSCs, neural stem cells; PCs, pericytes; TJ, tight junction; tv, transcript variant