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Fig. 2 | Fluids and Barriers of the CNS

Fig. 2

From: Human isogenic cells of the neurovascular unit exert transcriptomic cell type-specific effects on a blood-brain barrier in vitro model of late-onset Alzheimer disease

Fig. 2

Transcriptional alterations in LOAD or CON BCECs in mono- and co-culture with different NVU cell types. Comparison of mono- and co-cultures in the CON and LOAD NVU models by high-throughput multiplex qRT-PCR analysis of BBB markers expressed in BCECs (n = 3‑5 independent differentiations). (A) HiPSC‑derived CON and LOAD NVU models are displayed separately. The values of the logarithm of fold change (Log2FC) for each transcript is plotted on a heat map indicating up-regulated (red) and down-regulated (blue) transcripts. (B) Venn diagram showing the number of significantly regulated transcripts (p ≤ 0.05) shared between co-cultures with different brain cell types. CON and LOAD NVU models are displayed separately. (C) Volcano blot displaying Log2FC and p-values for each transcript in the CON NVU model (comparison of CON mono‑ and co‑cultures). Significantly up- (red) and down-regulated (blue) transcripts are shown. (D) Volcano blot displaying Log2FC and p-values for each transcript in the LOAD NVU model. (E) Venn diagram showing the number of significantly regulated transcripts shared between CON and LOAD co-cultures. BCECs, brain capillary endothelial-like cells; ACs, astrocytes; MGCs, microglia-like cells; NSCs, neural stem cells; PCs, pericytes, tv, transcript variant

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Fluids and Barriers of the CNS

ISSN: 2045-8118