Fig. 3

BTZ effect on P-gp-mediated Aβ42 transport in brain capillaries from hAPP mice. A Representative confocal microscopy images of brain capillaries isolated from WT mice, vehicle-treated hAPP mice, BTZ-treated hAPP mice, and BTZ/CSA-treated hAPP mice showing Fluorescein-β-amyloid1-42 (FL-hAβ42) accumulation in capillary lumens after a 1-h incubation (steady state; 5 μM FL-hAβ42). B Data after digital image analysis using ImageJ. Specific FL-hAβ42 fluorescence is the difference between total luminal FL-hAβ42 and FL-hAβ42 fluorescence with the P-gp-specific inhibitor PSC833 (5 μM). Columns represent mean ± SEM for 7 capillaries from one preparation (pooled tissue: WT: n = 20 mice; hAPP: n = 18 mice; hAPP-BTZ: n = 20 mice, hAPP-BTZ/CSA: n = 19 mice). Symbols indicate statistical groupings of pairwise comparisons adjusted for FDR. Columns that share a symbol do not significantly differ from each other