Fig. 7

Effects of age-dependent upregulation of Apoe and Apoj on brain function in APP-KI mice. A, B Violin plots of AD-related protein expression in the brain (A) and isolated brain capillaries (B) of 2-, 5-, and 12-month-old APP-KI mice. Twenty differentially expressed proteins were identified. Relative fold changes in protein expression (APP-KI/WT) in age-matched mice were calculated. Violin plot elements: center line, median; upper and lower lines, upper and lower quartiles. C, D Age-dependent changes in Apoe (C) and Apoj (D) levels in the brains of APP-KI mice. Bars represent the mean ± SD (n = 4–5). Plotted points represent individual values. E Correlation between age-dependent changes in Apoe and Gfap or C1qa in the brains of APP-KI mice. F Correlation between age-dependent changes in Apoj and Gfap or C1qa in the brains of APP-KI mice. G, H Correlation analysis of upregulation of Apoe or Apoj with 110 differentially expressed proteins in the brains of 5-month-old (G) and 12-month-old (H) APP-KI mice. Red circles indicate differentially expressed proteins (P < 0.05). I Correlation coefficients of differentially expressed proteins with Apoe or Apoj. J Network analysis of 16 differentially expressed proteins associated with Apoj expression using STRING and GO analyses. K, L Age-dependent changes in differentially expressed proteins associated with “mitochondria” in the brain (K) and brain capillaries (L) of APP-KI mice. Statistical significance was determined using one-way analysis of variance followed by Tukey’s post hoc test. Violin plot elements: center line, median; upper and lower lines, upper and lower quartiles