Fig. 5

Changes in protein expression related to BBB integrity in isolated brain capillaries of APP-KI mice. A Violin plots of protein expression identified in “ECM-receptor interaction (mmu04512)” and “laminin interactions (R-MMU-3000157)” in the isolated brain capillaries of 2-, 5-, and 12-month-old APP-KI mice. Relative fold changes in protein expression (APP-KI/WT) in age-matched mice were calculated. A total of 29 differentially expressed proteins were identified in the brain capillaries of APP-KI mice. Violin plot elements: center line, median; upper and lower lines, upper and lower quartiles. B Network analysis of these proteins using STRING and GO analyses. C, D Age-dependent changes in differentially expressed proteins associated with “basement membrane” (C) and “cell junction” (D) in APP-KI mice. Plotted points represent individual proteins. E–G Age-dependent expression of pericyte-selective proteins (E), astrocyte-selective proteins (F), and tight or adherens junction proteins (G) in brain capillaries of APP-KI mice. Relative fold changes in protein expression (APP-KI/WT) in age-matched mice were calculated, and P-values between age-matched APP-KI and WT mice were estimated using Welch’s t-test. Bars represent the mean ± SD values (n = 3–5). Plotted points represent individual values