Fig. 6

Capillary depleted brain lysate levels of nanobody construct 4 h after injection of a range of doses of biotinylated M1_{R56H, P96H, Y102H-}13A7-13A70-Nb80 in mice. N = 3 per dose, except for N = 2 at 1 nmol/kg and N = 2 for 1000 nmol/kg. Hill equation fit parameters were B_max = 3.94 nM, ED₅₀ = 5.6 nmol/kg, Hill coefficient = 1.6. Substrate inhibition fit parameters were B_max = 4.7 nM, ED₅₀ = 7.3 nmol/kg, K_i = 4877 nmol/kg