Skip to main content
Fig. 3 | Fluids and Barriers of the CNS

Fig. 3

From: Enhanced in vivo blood brain barrier transcytosis of macromolecular cargo using an engineered pH-sensitive mouse transferrin receptor binding nanobody

Fig. 3

BBB transcytosis of macromolecular cargos by M1R56H, P96H, Y102H and brain target engagement in vivo. A Diagram of the three nanobody construct including M1R56H, P96H, Y102H plus a tandem dimer of the P2X7 receptor binding nanobody 13A7 for brain target engagement. B Diagram of a control three nanobody construct including M1R56H, P96H, Y102H plus a tandem dimer of the human amyloid-beta binding nanobody Nb3 which has no known targets in the mouse brain. C Time course of capillary depleted lysate levels of biotinylated M1R56H, P96H, Y102H-13A7-13A7 (n = 3 per time point) after iv injection of 30 nmol/kg. D Time course of capillary depleted lysate levels of biotinylated M1R56H, P96H, Y102H-Nb3-Nb3 (n = 3 per time point) after iv injection of 30 nmol/kg. E–F Time course of blood levels of the biotinylated three nanobody constructs from the same mice as C, D

Back to article page

Fluids and Barriers of the CNS

ISSN: 2045-8118