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Fig. 1 | Fluids and Barriers of the CNS

Fig. 1

From: Enhanced in vivo blood brain barrier transcytosis of macromolecular cargo using an engineered pH-sensitive mouse transferrin receptor binding nanobody

Fig. 1

Binding and pH-dependent unbinding of M1 nanobody and histidine mutants to recombinant mTfR apical domain. Each nanobody was bound at multiple concentrations to 96 well ELISA plates coated with recombinant mTfR at pH 7.4, then washed at either pH 7.4 or pH 5.5. The original M1 has very little pH dependent unbinding, whereas the M1P96H mutant had an average of 1.46-fold more unbinding at pH 5.5 and the M1R56H, P96H, Y102H mutant had an average of 2.63-fold more unbinding at pH 5.5

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Fluids and Barriers of the CNS

ISSN: 2045-8118