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Table 2 Antibodies used for western blotting

From: Inhibition of serum- and glucocorticoid-induced kinase 1 ameliorates hydrocephalus in preclinical models

Target

Antibody

Species

Concentration

Target sequence

Verification method

TRPV4

Invitrogen PA5-77319

Rabbit

1:2000

CDGHQQGYAPKWRAEDAPL (Rat)

KO tissue, IP/IB with control IgG

SGK1

Upstate 07–315

Rabbit

1:500

C-GKSPDSVLVTASVK (Human)

IP/IB w/control IgG

p-SGK1

Abcam ab55281

Rabbit

1:200

Ser422 (Human)

control antigen

Upstate 36–002

Rabbit

1:500

Ser255/Thr256 (Human)

PDK activated SGK1 samples

p-NEDD4-2

Abcam Ab168349

Rabbit

1:500

Ser448 (Human)

293 T cell lysate

p-NDRG1

Cell Signaling 5482

Rabbit

1:2000

Thr346 (Mouse)

A204 cell lysate

  1. Antibodies included in this table were utilized in the experiments shown in Figs. 5 WB = western blot; KO = knockout; IP/IB = immunoprecipitation followed by western blot; TRPV4 = Transient receptor potential vanilloid 4; Serumglucocorticoid-induced kinase-1 = SGK1
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Fluids and Barriers of the CNS

ISSN: 2045-8118