Inhibition of serum- and glucocorticoid-induced kinase 1 ameliorates hydrocephalus in preclinical models

Table 1 Primer Pairs utilized for RT-PCR and qRT-PCR

Rattus norvegicus gene	Protein	Primer Sequences		Product Size (bp)
Rattus norvegicus gene	Protein	Forward Primer	Reverse Primer	Product Size (bp)
Gapdh	GAPDH	CCTGGAGAAACCTGCCAAGTAT	GACAACCTGGTCCTCAGTGTAG	103
Rps18	RPS18	CATGTGGTGTTGAGGAAAGCAG	TATTGTCGTGGGTTCTGCATGA	107
Trpv4	TRPV4	TCACCCTCACAGCCTACTATCA	GAGCCATCGACGAAGAGAGAAT	190
Aqp1	AQP1	GGCCAGCGAAATCAAGAAGAAG	CTCCAGTGGGTAATTGAAGCCT	118
ATP1A1	ATP1A1	ATTGCTGGTCTCTGTAACAGGG	ATGGAGAGCTGGTACTTGTTGG	206
ATP1B2	ATP1B2	AAGGAGTTCGTGTGGAATCCTC	AGGACGGCAGACATCATTCTTT	342
Sgk1	SGK1	CGGAGAGCTGTTCTACCATCTC	TAACCCAAGGCACTGGCTATTT	93

Primers included in this table were utilized for the experiments summarized in Fig. 4. Single band amplicon products were sequenced to verify correct gene amplification product. GAPDH = Glyceraldehyde 3-phosphate dehydrogenase; RPS18 = Ribosomal protein subunit 18; TRPV4 = Transient receptor potential vanilloid 4; AQP1 = Aquaporin 1; ATP1A1/B2 = ATPase Na + /K + Transporting Subunits Alpha 1/Beta 2; Serumglucocorticoid-induced kinase-1 = SGK1

ISSN: 2045-8118