Fig. 5

SI113-mediated changes in abundance of TRPV4, p-SGK1, and downstream targets of SGK1 in the choroid plexus of hydrocephalic (P394L) rats. A Representative immunoblots for lateral ventricle choroid plexus lysates probed for TRPV4 in normal and hydrocephalic animals, untreated or treated with SI113. Band intensities were normalized to Ponceau-S and the graphs represent the ratio of TRPV4 to total protein. Lysates were blotted in technical duplicates and n = 3 biological replicates. B Representative immunoblots for SGK1 and p-SGK1 (t256) in SI113 treated or untreated tissue from normal and hydrocephalic animals. Band intensities were normalized to Ponceau-S for total protein loading per lane, and the graphs represent the ratio of p-SGK1 to total SGK1 in the samples. Lysates were blotted in technical duplicates and n = 4 biological replicates. C Representative immunoblots for downstream targets of SGK1, p-NEDD4-2 and p-NDRG1 in lateral ventricle choroid plexus lysates in normal and hydrocephalic animals, untreated or treated with SI113. Band intensities were normalized to Ponceau-S and the graphs represent the ratio of the protein of interest to total protein. Lysates were blotted in technical duplicates and n = 2 biological replicates. Data represented as mean ± sem in all graphs. Significance values are displayed on the graphs and were determined by student’s unpaired t-test in GraphPad Prism 8.0