Fig. 5

Proteomic analysis of murine ChPE cells after exposure to rhIGF-1/rhIGFBP-3 or rhIGF-1. A-C. String network maps of significant changes in protein abundance (confidence level of 95%) in primary murine ChPE cells following exposure to rhIGF-1/rhIGFBP-3 (300 ng/ml) or rhIGF-1 (60 ng/ml), compared to cell culture medium only (Control), for 3.5 h. Colored based on KEGG and Reactome pathway enrichment. Unconnected nodes were removed from network maps. A. Increased proteins after rhIGF-1/rhIGFBP-3 stimulation. Red: spliceosome; blue: amyotrophic lateral sclerosis. B. Decreased proteins after rhIGF-1 stimulation. Red: formation of the cornified envelope; blue: post-translational protein phosphorylation; green: regulation of IGF transport and uptake by IGFBPs. C. Decreased proteins after rhIGF-1/rhIGFBP-3 stimulation. Red: signaling by receptor tyrosine kinases; blue: mRNA splicing - major pathway; green: mTORC1-mediated signaling; yellow: energy dependent regulation of mTOR by LKB1-AMPK. D-E. Significant changes in protein abundance when applying stricter significant criteria (fold-change ≥ 1.5 and ≤ -1.5, and Benjamini–Hochberg-corrected p-value ≤ 0.05, dotted lines), after rhIGF-1/rhIGFBP-3 (D) or rhIGF-1 (E) stimulation. GO terms based on David analysis for individual proteins are displayed