Fig. 4

Angiogenesis-related gene expression and IGF-1R activation in ChPE cells upon stimulation with rhIGF-1/rhIGFBP-3 or rhIGF-1. A. Outline of experimental in vitro setup. B. Western blot analysis of pERK1/2 and pPKB in primary murine ChPE cells following exposure to rhIGF-1/rhIGFBP-3 (300 ng/ml), rhIGF-1 (60 ng/ml) or cell culture media only (Control) for 15 min. Data from are presented as means ± SD (N = 4, pooled samples). C. Heat map, showing respective group mean absolute fold change and hierarchical clustering (N = 3–4), comparing gene expression of 84 genes related to angiogenesis using RT2 Profiler PCR Array in primary murine ChPE cells upon stimulation with rhIGF-1/rhIGBP-3 (300 ng/ml), rhIGF-1 (60 ng/ml) or culture medium only (Control) for 2 h. D - I. Individual analysis of selected target genes from the RT2 Profiler PCR Array, Bai1 (D), nitric oxide synthase 3 (Nos3) (E), plasminogen (Plg) (F), C-C motif chemokine 11 (Ccl11) (G), chemokine (C-X-C motif) ligand 2 (Cxcl2) (H) and epidermal growth factor (Egf) (I). Data are presented as means ± SD (N = 4). Differences in gene expression were analyzed using one-way ANOVA with post hoc Tukey for multiple comparisons of means, *P ≤ 0.05. P3-P8, postnatal day 3–8