Fig. 3

Temporal difference in gene expression in the ChP of preterm rabbit pups. A-I. QRT-PCR analysis of TTR (A), OTX2 (B), BMP4 (C), FOLR1 (D), ALB (E), BMP7 (F), AQP1 (G), AQP5 (H) and TGFβ3 (I) in ChP of preterm rabbit pups 5 h after s.c. administration of rhIGF-1/rhIGFBP-3 (8 mg/kg) or the corresponding vehicle solution at 0 (5), 24 (29) or 72 (77) postnatal age (corresponding time-point for termination within parentheses). Data are presented as means ± SD (N = 5–7). Differences in gene expression were analyzed using one-way ANOVA with post hoc Tukey test for multiple comparisons of means, *P ≤ 0.05, ***P ≤ 0.001