Fig. 7

β1 integrin blockade reduced the integrity of an endothelial monolayer. A A brain endothelial cell monolayer of bEnd3 cells in transwell cultures was measured 48 h after incubation with 10 µg/ml control antibody or anti-β1 integrin antibody. Permeability was measured with 4 kDa FITC-conjugated Dextran, 10 kDa Cascade Blue-conjugated Dextran and 40 kDa Texas Red-conjugated Dextran. *p < 0.05 and ***p < 0.001. B Representative images of ZO-1 immunostaining (red) and DAPI nuclear staining (blue) in endothelial cells after being incubated with control or anti-β1 integrin antibody. Scale bar = 25 μm. White arrows indicate points of junction disruption. C Quantification of the percentage of cells with disrupted junctions. ***p < 0.001. Note that β1 integrin blockade significantly increased the permeability of the endothelial monolayer to all three sizes of dextrans and induced disruptions in the ZO-1 staining pattern