Fig. 1
Chronic mild hypoxia (CMH)-induced upregulation of fibronectin and α5 integrin is greater in aged brain. A Frozen brain sections taken from young (8–10 weeks) or aged (20 months) mice exposed to normoxia or 7 days hypoxia (8% O2) were stained for fibronectin or α5 integrin. Images were captured in the midbrain. Scale bar = 50 μm. B–D Quantification of fibronectin (B), α5 integrin (C), or β1 integrin (D) in the midbrain after 0, 4, 7 and 14-days hypoxia. All results are expressed as the mean ± SEM (n = 4–6 mice/group). *p < 0.05, **p < 0.01, ***p < 0.001. Note that CMH-induced upregulation of fibronectin and α5 and β1 integrin was greater in aged brain