Fig. 4

Dark-light phase fluctuation in rat choroid plexus transporter expression. A A schematic choroid plexus epithelial cells with transporters proposed involved in CSF secretion illustrated on the respective membranes. B Gene expression of these transporters quantified as transcripts per million (TPM) following RNAseq of acutely excised choroid plexus tissue obtained in the light phase (ZT 8 h, yellow bars) and the dark phase (ZT 20 h, grey bars), n = 6 of each. Transcripts left of the dashed vertical line are those of transporters expressed in the luminal membrane. NBCe2 the electrogenic Na⁺,HCO₃^- cotransporter 2, AQP1 aquaporin 1, NKA the Na⁺/K⁺-ATPase.α₁ subunit, NKCC1 the Na⁺, K⁺, 2Cl^- cotransporter, NCBE the Na⁺-driven Cl^-/HCO₃^- exchanger, NBCn1 the electroneutral Na⁺,HCO₃^- cotransporter 1, and AE2 the anion exchanger 2, ZT zeitgeber time. Insert illustrates the expression level of TRPV4 transient receptor potential vanilloid 4 ion channel and WNK1 lysine-deficient protein kinase 1. Data are shown as mean ± SD and statistical significance was evaluated with Welch ANOVA test. **P < 0.01, *P < 0.05, ns; not significant