Fig. 2

Dark-light phase rodent fluctuations in ICP, blood pressure and heart rate . A Continuous telemetric ICP recordings from freely moving, non-anaesthetized rats obtained in the light phase (ZT 0–12 h) and the dark phase (ZT 12–24 h), n = 9. B Average ICP across the light and dark phases illustrated in the left panel, with the intra-rat ∆ICP illustrated in the right panel, n = 9. C The ICP at ZT 8–10 h and ZT 20–22 h (left panel) and the intra-rat ∆ICP (right panel), n = 9. D Continuous telemetric MAP recordings from freely moving, non-anaesthetized rats obtained in the light phase (ZT 0–12 h) and the dark phase (ZT 12–24 h), n = 9. E Average MAP across the light and dark phases illustrated in the left panel, with the intra-rat ∆MAP illustrated in the right panel, n = 9. F The MAP at ZT 8–10 h and ZT 20–22 h (left panel) and the intra-rat ∆MAP (right panel), n = 9. G Continuous telemetric heart rate recordings from freely moving, non-anaesthetized rats obtained in the light phase (ZT 0–12 h) and the dark phase (ZT 12–24 h), n = 9. H Average heart rate across the light and dark phases illustrated in the left panel, with the intra-rat ∆heart rate illustrated in the right panel, n = 9. I The heart rate at ZT 8–10 h and ZT 20–22 h (left panel) and the intra-rat ∆heart rate (right panel), n = 9. J MAP, heart rate (K), and ICP (L) obtained in video-based identified time points of voluntary sedentary periods in windows spanning ZT 6–11 h in the light phase and ZT 18–23 h in the dark phase. Measurements in left panels and intra-rat delta values in right panels, n = 6. ICP intracranial pressure, MAP mean arterial pressure, HR heart rate, ZT zeitgeber time. Data are shown as mean ± SD and statistical significance was evaluated with paired two-tailed t-test (left panels) and one sample t-test (right). ***P < 0.001, **P < 0.01, *P < 0.05, ns; not significant