Table 2 Details of fluorophores used
Fluorophore | Isotype | Catalogue number | Company | Dilution |
|---|---|---|---|---|
Alexa fluor488 | Anti-mouse IgG | 715-545-151 | Jackson | 1:800 |
Alexa fluor568 | Anti-rabbit IgG | A11011 | Life Technologies | 1:1000 |
Alexa fluor647 | Anti-rat IgG | 712-605-153 | Jackson | 1:1200 |
Atto488 | Anti-mouse IgG | 62197 | Sigma-Aldrich | 1:800 |
Atto488 | Anti-rabbit IgG | 18772 | Sigma-Aldrich | 1:800 |
CF568 | Anti-mouse IgG | 20105 | Biotum | 1:800 |
CF568 | Anti-rabbit IgG | 20098 | Biotum | 1:800 |
Streptavidin Alexa fluor647 | Biotin | S32357 | Molecular Probes | 1:1000 |
Streptavidin HRP | Biotin | S911 | Invitrogen | 1:1000 |