Development of a method for isolating brain capillaries from a single neonatal mouse brain and comparison of proteomic profiles between neonatal and adult brain capillaries

Table 2 Ratio of protein expression in nBC fraction to aBC fraction

Protein	nBC-to-aBC ratio
	Fold	SD	Significance
ABC transporters
Abcb1a/Mdr1a	0.189	0.060	***
Abcc4/Mrp4	0.247	0.065	***
Abcg2/Bcrp	0.471	0.072	***
SLC transporters
Slc1a4/Asct1	3.94	0.32	***
Slc2a1/Glut1	0.128	0.149	***
Slc3a2/4F2hc	0.763	0.106	**
Slc7a1/Cat1	1.80	0.11	***
Slc7a5/Lat1	1.09	0.15
Slc16a1/Mct1	2.48	0.14	***
Slc22a8/Oat3	1.13	0.23
Slc27a1/Fatp1	0.910	0.261
Slco1a4/Oatp1a4	0.159	0.066	***
Slco1c1/Oatp1c1	1.54	0.20	**
Receptors
Transferrin receptor	4.11	0.30	***
Insulin receptor	0.804	0.228
Lrp1	1.01	0.07
FcRn large subunit	0.484	0.064	***
Tight junction and adherens junction proteins
Claudin-5	1.49	0.27	*
Occludin	0.258	0.111	***
Jam1	0.461	0.079	***
Cadherin-5/VE-cadherin	0.351	0.089	***

Each nBC-to-aBC enrichment ratio represents the mean and standard deviation (SD) (n = 4). *p < 0.05, **p < 0.01, and ***p < 0.001 represent significant differences between the neonatal brain capillary (nBC) and adult brain capillary (aBC) fractions assessed by the Welch t-test

ISSN: 2045-8118