Development of a method for isolating brain capillaries from a single neonatal mouse brain and comparison of proteomic profiles between neonatal and adult brain capillaries

Table 1 Enrichment ratios of proteins in the nBC fraction to the whole brain lysate

Protein	nBC-to-brain enrichment ratio
	Mean	SD	Significance
Brain capillary endothelial cells
Abcb1a/Mdr1a	17.6	5.5	***
Abcc4/Mrp4	8.85	13.63	***
Abcg2/Bcrp	6.75	1.77	***
Slc2a1/Glut1	20.1	4.5	***
Slc7a5/Lat1	5.23	0.79	***
Slc22a8/Oat3	6.63^a
Slco1c1/Oatp1c1	11.5	3.6	***
Tie2/Tek	nBC only
Tfr1/Tfrc	18.0	4.4	***
Claudin-5	17.5	7.6	***
Occludin	nBC only
JAM1	5.93	1.61	***
Esam	15.3	7.7	***
Pecam1	13.4	8.7	***
vWF	20.8	17.9	***
Neurons
Synaptophysin	0.0861	0.0131	***
Map2	0.141	0.023	***
Astrocytes
Gfap	0.885	0.235
Slc1a2/Eaat2	0.192	0.021	***
Slc1a3/Eaat1	0.510	0.0495	***
Pericytes
Pdgfrb	16.2	9.0	***
Muc18/Mcam	5.51	0.51	***
Oligodendrocytes
Cnp	0.275	0.021	***
Microglia
Progranulin	2.05	0.23	***
Cdk11b	2.03	1.32	***
Vascular smooth muscle cells
Acta2	3.05	1.04	*
Taglin	1.02^a
Myh11	6.80	2.56	***

Each nBC-to-brain enrichment ratio represents the mean and standard deviation (SD) (n = 4), *p < 0.05 and ***p < 0.001 represent significant difference between the neonatal brain capillary (nBC) fraction and whole brain lysate assessed by the paired-samples t-test. nBC only, protein expression was detected only in the nBC fraction. ^aDetected in all four nBC fractions, but only in two of four whole brain samples

ISSN: 2045-8118