Fig. 3

Brain region-wise analysis of tracer distribution. TSCs for all anesthetic conditions represented by mean (solid or broken lines) and confidence interval (shaded area) in neocortex (A), brainstem (B), striatum (C) and thalamus (D). Brain region location is indicated in blue in volume renderings. E 3D visualization of time to maximum, F 3D visualization of time to start for MED and MED+AZE. Boxplots display time to start (G), time to maximum (H), maximum signal (I), and AUC (J) of neocortex. Animal numbers for plots in A–D and G–J were ISO n = 8; MED n = 7; ISO+MED n = 8; ISO+AZE n = 10; MED+AZE n = 8; ISO+MED+AZE n = 8. Kruskal–Wallis test showed an effect of experimental condition for all TSC parameters (time to start: p = 0.011; time to maximum: p = 0.007; maximum signal: p < 0.001; area under the curve: p < 0.001), the post-hoc analysis was performed using Mann–Whitney-U test. Significant differences are indicated in the graphs. BL: baseline. * p < 0.05, ** p < 0.01, *** p < 0.001