Fig. 5
Mouse effector/memory CD4+ T cells can migrate across the non-stimulated and cytokine stimulated BCSFB in vitro. (A) The migration of MOG35 − 55-specific T helper cell subsets (Th0, Th1, Th2, Th17) across unstimulated pmCPEC from the basolateral to apical side towards 100 ng/mL CXCL12 after 8 h is shown. The number of T cells added to the assay (imput = 4 × 105 cells) was set as 100% and the numbers of migrated T cells was assessed by flow cytometry. Bars show the mean % ± SD of two experiments with three filters per condition. (B) The migration of MOG35 − 55-specific T helper cell subsets (Th0, Th1, Th2, Th17) across unstimulated or either TNFα or IFNγ (10 ng/mL, 24 h) stimulated pmCPEC from the basolateral to apical side after 8 h is shown. The number of T cells added to the assay (imput = 4 × 105 cells) was set as 100% and the numbers of migrated T cells was assessed by flow cytometry. Bars show the mean % ± SD of two experiments with three filters per condition. Statistical analysis: (A) one-way ANOVA and (B) two-way ANOVA (p < 0.05 = *, p < 0.01 = **, p < 0.001 = ***, p < 0.0001 = ****)