Fig. 4

The numbers of CD45⁺ cells in the CSF increase after aEAE induction. (A) CSF tapping was performed by puncture of the cisterna magna of PBS perfused mice. On average a total of 15 µl of CSF was harvested and pooled from three mice, sedimented via cytospin and double-stained for CD45 (green) and CD4 (red). The CSF was collected from healthy mice and mice suffering from aEAE before onset of the disease (d 13 p.i.), at peak (d 18 p.i.) and during the chronic phase (d 30 p.i.). Scale bars = 50 μm. (B) Quantification of the absolute numbers of CSF derived CD45⁺CD4⁺ (yellow) and CD45⁺ (green) immune cells on the slides from healthy mice and mice suffering from aEAE.