Fig. 3

The composition of the immune cells in choroid plexus, brain, and spinal cord during aEAE progression. Absolute numbers of B220⁺ B cells (A) and CD11b⁺ myeloid subsets including Ly6G⁺ neutrophils, Ly6C^hi monocytes (B), CD11c⁺ dendritic cells and F4/80⁺ macrophages (C) per mouse in the choroid plexus (ChP), brain and spinal cord (SC) of healthy mice and mice suffering from aEAE, were acquired by flow cytometry as shown. The graphs show means ± SEM of six independent experiments. Significant differences between ChP and SC or ChP and brain are shown in brown and black stars, respectively. Statistical analysis: (A-C) one-way ANOVA (p < 0.05 = *, p < 0.01 = **, p < 0.001 = ***, p < 0.0001 = ****)