Fig. 2

The choroid plexus harbors high numbers of CD4⁺ and CD8⁺ T cells as well as CD4⁺CCR6⁺ T cells when compared to brain and spinal cord. (A) Absolute numbers of CD45^hi CD4⁺ and CD45^hi CD8⁺ T cells per mouse in the choroid plexus (ChP), brain and spinal cord (SC) of healthy mice and mice during progression of aEAE as determined by multi-color flow cytometry. The graphs show means ± SEM of six independent experiments as outlined in Supplementary Fig. 1. (B) Contour plots for CD4 and CCR6 of CD45^hi immune cells isolated from ChP, brain and SC are shown. Numbers depict percentages of cells detected in the respective quadrant. Data are representative for two experiments (healthy, pre-clinical, before onset) and three experiments (peak, chronic and late chronic stages). Significant differences between ChP and SC or ChP and brain are shown in brown and black stars, respectively. Statistical analysis: one-way ANOVA (p < 0.05 = *, p < 0.01 = **, p < 0.001 = ***, p < 0.0001 = ****)