Fig. 1

CD45⁺ immune cell subsets change after induction of aEAE in brain, spinal cord, and choroid plexus. Immune cells were isolated from the choroid plexus (ChP) of the fourth and both lateral ventricles, brains, and spinal cords (SC) of healthy C57BL/6J mice and C57BL/6J mice suffering from aEAE at the different disease time points as outlined in Supplementary Fig. 1 and analyzed by multi-color flow cytometry for CD45⁺ immune cell subsets. (A) Representative dot plots (SSC versus CD45) show the ungated events acquired from ChP, brain and SC and the gating strategy for CD45⁺ immune cells. Gate bordered by the red line depicts 100% of CD45⁺ immune cells, which is divided by the black line into two gates for CD45^int and CD45^hi immune cells subsets. Numbers within the gates depict the percentages of CD45^int and CD45^hi immune cells, respectively. (B) Quantification of the percentages of CD45^int and CD45^hi immune cell subsets in the ChP, brain and SC during EAE. (C) Absolute numbers of CD45⁺ as well as CD45^int and CD45^hi immune cell subsets per mouse in the ChP, brain and SC in healthy C57BL/6J mice and at the investigated time points during aEAE are shown. The graphs in B and C show means ± SEM of six independent EAE experiments as outlined in Supplementary Fig. 1B. Significant differences between ChP and SC or ChP and brain are shown with brown and black stars, respectively. Statistical analysis: one-way ANOVA (p < 0.05 = *, p < 0.01 = **, p < 0.001 = ***, p < 0.0001 = ****)