Fig. 9

Myelination is recovered in WTs after microglia repopulation at P20, but it worsens hypomyelination phenotype in prh mutants. A Low magnification 4 × images of CNPase (purple) and DAPI (blue) stained in P20 WT and prh brains with and without PLX5622 treatment. Arrows indicate the myelination in corpus callosum (CC) where myelination is quantified. Scale bar = 1000 μm. LV: Lateral ventricle. HIP: Hippocampus, B Quantification of myelination density at P18-21 shows that myelination is comparable between PLX-treated WT mice within thirteen days after withdrawal of PLX5622 treatment. PLX-treated prh animals has significantly lower myelination density than untreated prh mutants. P18 (triangles), P20 (circles), and P21 (rectangle). Stats: two-way ANOVA followed by Tukey’s test, n > 5 in each group, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05