Fig. 8

Characteristics of microglia after repopulation in the white matter of WT and prh brains at P20. A P20 somatosensory cortex double stained with ApoE (green) and IBA1 (red). Dotted lines indicate the border of grey (GM) and white matter (WM). Arrowheads: ApoE⁺ microglia. B The raw density of immature ApoE⁺ Iba1⁺ microglia in white matter shows no significant difference in density between treatment or genotype groups in white matter at P20 (two-way ANOVA followed by Tukey’s test, n ≥ 3 in each group). C Ratio of immature ApoE⁺ microglia out of total IBA1⁺ microglia in white matter. D P20 somatosensory cortex double stained with CD86 (green) and IBA1 (red). Dotted lines indicate grey matter (GM) layers and white matter (WM) layer. Arrowheads: CD86⁺ microglia. E Raw density of pro-inflammatory CD86⁺ IBA1⁺ microglia in white matter shows no significant difference between treatment and genotype groups at P20 (two-way ANOVA followed by Tukey’s test, n ≥ 3 in each group). F Ratio of pro-inflammatory CD86⁺ microglia out of total IBA1⁺ microglia in white matter. Scale bars: 100 μm.