Fig. 7

Cessation of PLX5622 treatment successfully repopulates microglia by P20 in both WTs and prh mutants. A Low magnification 10 × images of IBA1 (red) stained in P20 WT and prh brains with and without PLX5622 treatment. Dotted lines indicate borders of cortical layers II-VI, V-VI, and white matter (WM). Scale bar = 100 um. B IBA⁺ microglial densities in cortical layers II-IV (top), V-VI (middle), and white matter (bottom) shows that thirteen days post withdraw of PLX5622 treatment allows successful microglial repopulation to levels of those comparable within the same genotype group in cortical grey matter (layers II-IV and V-VI) and white matter of the somatosensory cortex (n ≥ 3 in each group). C High magnification (60x) images of amoeboid-shaped IBA1⁺ (purple) microglia at P20. Scale bar = 10 μm. D Amoeboid-shaped IBA1⁺ microglia densities in white matter are comparable between treatment and genotype groups at P20 E Ratio of amoeboid-shaped microglia among IBA1⁺ microglia in white matter at P20