Fig. 5

Ventricular size is enlarged, and parenchyma volume is decreased in PLX-treated prh mutants. A 3D reconstruction of fluid sensitive MR images. Blue: lateral ventricles. Green: third ventricles. Red: fourth ventricles and pineal recesses. Scale bar = 2 mm. B (Left) Raw ventricular volume (sum of lateral ventricles, third ventricle, fourth ventricle, and pineal recess) and its ratio to total brain volume shows enlarged ventricles in prh mutant animals compared to WT animals, with PLX-treated prh mutants also having significantly larger ventricular volume when compared to untreated-prh. C 3D reconstruction of MR images showing total brain volume. The total brain is marked purple. Scale bar = 2 mm. D Raw parenchyma volume, calculated by subtracting ventricular volume from total brain volume, shows prh mutants have significantly smaller parenchyma compared to WTs. E (Left) Raw total brain volume shows no significant difference among the groups. (Right) Relative brain volume to body weight (BW) shows both untreated and PLX-treated prh mutants have significantly higher relative brain ratio compared to WTs. Stats: two-way ANOVA followed by Tukey’s test, n > 6 in each group, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05