Fig. 4

PLX5622 does not improve myelination in prh, rather it decreases white matter myelination in WT. A Low magnification 10 × images of CNPase (purple) and DAPI (blue) stained sections including somatosensory cortex in P8 WT and prh mice with and without PLX5622 treatment. Arrows indicate the myelination of corpus callosum where quantification is performed. Scale bar = 1000 μm. CC: Corpus callosum. LV: Lateral ventricle. HP: hippocampus. B Myelination density in white matter (WM), is significantly lower in PLX-treated WT, untreated-prh, and PLX- treated WT, compared to untreated WT at P8. C Pan-oligodendrocyte lineage marker OLIG2 (green) and DAPI (blue) stained in P8 WT and prh brains with and without PLX5622 treatment. White dotted area indicates white matter (WM). Scale bar = 100 um. D OLIG2 positive cells density is significantly lower in PLX-treated WT (*p = 0.011) as well as in untreated prh (****p = 0.0001). Stats: two-way ANOVA followed by Tukey’s test, n > 10 in B, n > 8 in D in each group, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05