Fig. 2

P8 microglial profiling with and without PLX5622 treatment. A P8 somatosensory cortex double stained with ApoE⁺ (green) and IBA1⁺ (red). Dotted lines indicate the border of grey (GM) and white matter (WM). Arrowheads: ApoE⁺ microglia. Scale bars = 100 μm. B The raw density of ApoE⁺ IBA1⁺ microglia is significantly reduced in PLX-treated mice compared to untreated mice. C Ratio of immature ApoE⁺ microglia out of total IBA1⁺ microglia in white matter. D P8 somatosensory cortex double stained with CD86 (green) and IBA1 (red). Dotted lines indicate grey matter layers and white matter layer. Arrowheads: CD86⁺ microglia. Scale bars = 100 μm. E Raw density of pro-inflammatory CD86⁺ IBA1⁺ microglia in white matter is significantly decreased in PLX-treated prh when compared to untreated mice. CD86⁺ IBA1⁺ microglial density is also reduced in PLX-treated WT when compared to untreated mice. F Ratio of pro-inflammatory CD86⁺ microglia out of total IBA1⁺ microglia in white matter. Stats: two-way ANOVA followed by Tukey’s test, n ≥ 10 in each group, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05