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Table 3 Retinal thickness in control and GNAZ-KD mice

From: Blue light exposure collapses the inner blood-retinal barrier by accelerating endothelial CLDN5 degradation through the disturbance of GNAZ and the activation of ADAM17

 

Treatment

Control

GNAZ-KD 1

Total retinal thickness

231.3 ± 0.9

218.4 ± 1.7***

Retinal sub-layers analysis

  

IPL-to-INL

  

mean thickness (μm)

97.7 ± 0.7

100.1 ± 1.2

% of total retina

42.5 ± 0.2

45.9 ± 0.8***

OPL-to-ONL

  

mean thickness (μm)

75.2 ± 0.7

70.8 ± 1.3**

% of total retina

32.8 ± 0.3

32.4 ± 0.4

IS/OS

  

mean thickness (μm)

46.1 ± 0.8

35.5 ± 1.3***

% of total retina

19.4 ± 0.3

16.2 ± 0.5***

RPE

  

mean thickness (μm)

12.3 ± 0.1

12.0 ± 0.2

% of total retina

5.3 ± 0.1

5.5 ± 0.1

  1. The right eye of the mice received 1 μL mixture of GNAZ shRNA (20 ng per eye) and jetOPTIMUS transfection reagent by ITV injection, and sham treatment was performed on the left eye. An ophthalmology examination battery was conducted on day 5 after ITV injection. The mice were then euthanized. Their eyeballs were isolated, and homogenates were prepared. GNAZ knockdown in vivo was validated by immunoblotting
  2. Retinal thickness was computed automatically by the software
  3. Data are expressed as mean ± S.E.M. **p < 0.01, ***p < 0.001, indicates statistical difference from the control group. The N values are listed in Additional file 1: Table S1
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Fluids and Barriers of the CNS

ISSN: 2045-8118