Fig. 5From: Direct association with the vascular basement membrane is a frequent feature of myelinating oligodendrocytes in the neocortex Remyelination reestablishes vascular association of oligodendrocytes (A) Schematic of the experimental timeline and schematic overview and locations of the microscopy images shown in B and D (black box). (B) CNPase labeling of the motor cortex reveals that 5 weeks of cuprizone treatment leads to near complete demyelination compared to control. Red boxes indicate the zone of quantification in layer 2/3, a dotted line denotes the cortex surface. (C) Quantifications of the oligodendrocyte density after 5 weeks of demyelination in layer 2/3, compared to age matched controls (12 week old mice, unpaired t-test). (D) Quantifications in layer 2/3 show that vOLs are strongly reduced (unpaired t-test). (E) Example overview images of the cortex from age matched control and remyelinated mice that were labeled with a CNPase antibody. In the remyelinated cortex, myelin still appears less homogeneous compared to control. Red boxes indicate the area of quantification in layer 2/3. (F) Quantifications in layer 2/3 show that after 7 weeks of remyelination, total oligodendrocyte density is still reduced in motor cortex compared to age matched control mice. Age of mice at time of analysis is 22 weeks. Unpaired t-test, p = 0.001. (G) Example of a vOL in motor cortex (arrow head) after 7 weeks of remyelination. (H) Quantifications of vOL density during remyelination show a reduction compared to control. Single data points represent data from one animal. Unpaired t-test, p = 0.024, n = 4 animals for control and for remyelination. (I) Quantifications reveal that the percentage of vOLs during remyelination is comparable to the control motor cortex. Unpaired t-test, p = 0.8, n = 4 animals for control and for remyelination. Single data points in C, D, F, H and I represent averaged data from one animal.Back to article page