Fig. 8

Common and unique DEGs with altered DNA methylation in poststroke BBB in young and old mice A Pearson correlation (left) between DMRs common to poststroke BBB in old (18 months) and young (6 months) mice, regardless of genomic location, with percent methylation difference of old post-TE stroke and young post-TE stroke on the x- and y-axes, respectively. Statistically significant DMRs with methylation changes in the same direction (e.g., hypermethylated in both groups) are blue, statistically significant DMRs with methylation changes in the opposite direction (e.g., hypermethylated in aging post-TE stroke and hypomethylated in young post-TE stroke) are red. Venn diagram (right) demonstrates the number of common DMRs between the old and the young post-TE stroke mice. Only statistically significant DMRs are represented, with direction of change (i.e., hypermethylation or hypomethylation) not considered. B Pearson correlation (left) between common DEGs for old and young post-TE stroke mice, with log2 fold change of DEGs in old post-TE stroke mice and young post-TE stroke mice on the x- and y-axes, respectively. Statistically significant DEGs regulated in the same direction (e.g., upregulated transcript expression in both groups) are blue, and statistically significant DEGs regulated in opposite directions (e.g., upregulated in aging post-TE stroke mice and downregulated in young post-TE stroke mice) are red. Venn diagram (right) showing the number of common DEGs between old post-stroke TE mice and young post-TE stroke mice. Data reflects statistically significant DEGs, with direction of change, i.e., upregulation or downregulation, not considered. C Heatmaps demonstrating examples of genes with DMRs regulated similarly across old post-TE stroke and young post-TE stroke (top) and their changes in gene expression (bottom). D Heatmaps depicting examples of genes with DMRs regulated differentially between the two experimental groups (top) and their changes in gene expression (bottom). E Tables demonstrating DEGs with altered methylation unique to poststroke BBB of young (6 months) mice. Genes are categorized based on their cellular process. F Tables demonstrating DEGs with altered methylation unique to poststroke BBB in old (18 months) mice, with genes classified by their cellular process. Genes were selected based on their statistical significance and their importance in endothelial cell function