Fig. 7

tPA is present in brain and liver endothelial cells. A Epifluorescence images of brain cortex and liver slices from C57BL/6 adult mice (Scale bar = 50 µm, zooms = 10 µm) revealing tPA (red), CD31 (green) and cells nuclei (DAPI, blue). tPA was observed in endothelial cells (arrow) in both the brain cortex and liver. Lumen of vessels was represented by stars. B Hepatocytes do not express tPA, but liver endothelial cells do. Representative photomicrographs of liver tissue sections subjected to in situ hybridization to reveal plat mRNA (mRNA encoding for the gene tPA; green), phalloïdine (hepatocyte marker; grey) and CD31 (red) staining; scale bar 100 µm and 50 µm in the magnified inserts. Images were digitally captured using a camera (CoolSNAP; Photometrics) and/or with an inverted confocal microscope (SP5, Leica). Images were visualized respectively with Metavue 5.0 software (Molecular Devices, USA) and LAS AF lite software (LEICA)