Fig. 7
CU06-1004 reduces neuronal loss and cognitive deficits in aged mice. A Neuronal nuclei (NeuN) were visualized using immunofluorescence staining in brain tissues from 6-week-old (young), 24-month-old vehicle-treated (old-veh), and 24-month-old CU06-1004–treated mice (old-1004). Scale bar = 50 µm. B The number of NeuN-positive cells/mm2 was calculated in cortical and hippocampal CA1 regions in young, old-veh, and old-1004 mice. C–F Behavior tests were conducted on 23-month-old mice. D Rotarod test; E Wire hang test; F T-maze test. Mice were given oral injections of olive oil (vehicle) or CU06-1004 (10 mg/kg) for 6 months, and behavioral tests were performed separately at 23 months (n = 10–12 mice per group). #P < 0.05, ###P < 0.001 vs. young. *P < 0.05, ***P < 0.001 vs. old-veh. B Results are presented as the mean, and error bars represent the standard deviation. D–F Results are presented as the mean, and error bars represent the standard error of the mean