Fig. 5

CU06-1004 administration rescued impaired blood–brain barrier integrity and reduced tight junction protein coverage in aged mice. A Confocal microscopic images of plasma IgG extravasation as a marker of blood–brain barrier disruption. Scale bar = 50 µm. B Quantitative analyses of mean IgG mean intensity in cortical and hippocampal regions in 6-week-old (young), 24-month-old vehicle-treated (old-veh), and 24-month-old CU06-1004–treated mice (old-1004). C Double immunostaining of claudin-5 and CD31 (left) and occludin and CD31 (right). Representative images show the abundance of claudin-5 (green) and occludin (green) in cerebral vessels of young, old-veh, and old-1004 mice. Scale bar = 20 µm. D Quantitative analysis of the claudin-5/CD31 and occludin/CD31 ratios (n = 6–10 per group). All data were analyzed with one-way analysis of variance, followed by Tukey’s multiple comparison test. ^###P < 0.001 vs. young. *P < 0.05 vs. old-veh. n.s., not significant. Results are presented as the mean, and error bars represent the standard deviation